LGALS9 and precursor B-cell acute lymphoblastic leukemia: To determine whether the increased surface expression of GAL-9 required continued exposure to adipocyte-secreted factors, human B-ALL cells were cultured in unconditioned medium or CM for 3 days, washed, and cultured in unconditioned for an additional 3 days with GAL-9 surface expression assessed before and after the removal of CM.