Quantitative RT-PCR (RT-qPCR) was performed to quantify CPA3, tryptase, and B2MG in 17 nodes with no evidence of metastasis (HN0), 20 nodes with pre-metastasis (HN1), 20 nodes with early-metastasis (HN2), 21 nodes with overt-metastasis (HN3), and 13 control nodes with non-tumor dogs. This evidence concerns the gene CPA3 and neoplasm.