Unlike previous reports (40, 42–45), we observed that LINC00324 and LOC100507053 expression were correlated with ESCC patients’ those who smoked tobacco, consumed alcohol, hot beverages compared to healthy individuals, suggesting that these factors may modulate the expression of LINC00324 and LOC100507053 in ESCC patients, but these factors were not correlated within the ESCC patients. Here, LINC00324 is linked to esophageal squamous cell carcinoma.