In the present study, flutamide was found to ameliorate DHEA-induced impairment of follicle development and ovulation, which further supported the direct inhibition of androgen excess on follicle development and suggested the involvement of AR signalling in androgen-induced lipid metabolism and fatty acid synthetic disorders in granulosa cell, which provides new insights into the role of AR-driven metabolic dysfunction of granulosa cells in PCOS pathogenesis. Here, AR is linked to polycystic ovary syndrome.