Moreover, PPAR signaling pathway were enriched in KEGG analysis, suggesting that metabolic state in PCOS granulosa cells evidently differed from that in control, and the enrichment of DEGs in ovarian steroidogenesis may further support the abnormal secretion of steroid hormones by granulosa cells in women with PCOS (Figure 1D). This evidence concerns the gene PPARA and polycystic ovary syndrome.