Besides, 10 hub genes, including SREBF1, HMGCR, FASN, SCD, INSIG1, FADS2, SCD5, ACSS2, LDLR, and LSS, were identified by Cytohubba (Figure 2E), and the RT-qPCR result indicated the decreased expression of these 10 hub genes, among which SREBF1, SCD, INSIG1, FADS2, ACSS2, and LDLR were significantly decreased in granulosa cells from women with PCOS, which was consistent with the RNA-Seq result (Figure 2F). The gene discussed is FASN; the disease is polycystic ovary syndrome.