Similar to TGF-β1 (Lane 2, Figures 3A,B), the plasma EVs isolated from the high liver fibrosis patients induced increased expressions of α-SMA, Collagen I, β-catenin, cyclin-D1 and c-myc at both mRNA and protein levels in LX-2 cells (Lane 4, Figures 3A,B), as compared with those of the plasma EVs isolated from the control patients (Lane 3, Figures 3A,B). The gene discussed is CCND1; the disease is Hepatic fibrosis.