Similar to TGF-β1 (Lane 2, Figures 3A,B), the plasma EVs isolated from the high liver fibrosis patients induced increased expressions of α-SMA, Collagen I, β-catenin, cyclin-D1 and c-myc at both mRNA and protein levels in LX-2 cells (Lane 4, Figures 3A,B), as compared with those of the plasma EVs isolated from the control patients (Lane 3, Figures 3A,B). This evidence concerns the gene TGFB1 and Hepatic fibrosis.