Moreover, treatment with DA1 suppressed MAM hyperconnectivity in the AD mice, as demonstrated by the reduced number of PLA-positive puncta in 6-month-old 5XFAD AD mouse brains following staining with anti-SigmaR1 and anti-VDAC or anti-ATAD3A and anti-FACL4 antibodies (Fig. 4b, Supplementary Fig. 6c). This evidence concerns the gene VDAC1 and Alzheimer disease.