Quantitative PCR (qPCR) of the tumor tissue samples revealed that doxycycline induced Meflin expression in Col1a1+ stromal cells, representing CAFs, in tumors developed in Meflin-TO mice administered doxycycline, but not in control mice that lack the Meflin-Cre allele (Fig S7B). This evidence concerns the gene COL1A1 and neoplasm.