RAB5A and early-onset autosomal dominant Alzheimer disease: To determine whether Alzheimer’s disease risk factors of age and genetic mutations resulting in increased Aβ are additive to their influence on syndapin-2 and Rab5 expression, we also performed quantitative immunoblotting on brains obtained from 4- and 12-months-old APP-PS1 mice (Fig. 5C1–3, Supplementary Fig. 6B).