The results displayed that the number of Ki-67-positive cells in ESCCAL-1 overexpression group (OE-AL-1) was significantly increased when compared to control group (OE-NC), which further indicated that overexpression of ESCCAL-1 promoted the proliferation of ESCC cells in vivo (Fig. 7D). Here, MKI67 is linked to esophageal squamous cell carcinoma.