Meanwhile, flow cytometry and western blot analysis revealed that the effects of ESCCAL-1 overexpression on cell cycle and cell cycle regulators (CDK4, CCND1, and CDKN1A) were abolished, at least in part, by knockdown of Gal-1 in ESCC cells (Fig. 6C, D). The gene discussed is CDKN1A; the disease is esophageal squamous cell carcinoma.