To explore the underlying mechanism by which OTUD6A induces PCa progression, we identified its binding proteins with an immunoprecipitation (IP)-mass spectrometry (MS) approach in two CRPC cell lines VCaP and PC-3; VCaP cells are positive for AR expression and show a growth response in the presence of androgen27, whereas PC-3 cells are deficient in AR (Supplementary Fig. 4a, b). This evidence concerns the gene AR and posterior cortical atrophy.