Brg1 was previously reported to reside on the proximal promoter region of AR and to activate AR and PSA in prostate cancer;33,34 in our study, we found that Brg1 indeed transcriptionally regulated AR expression through qPCR and ChIP-qPCR assays (Supplementary Fig. 5f, g). This evidence concerns the gene KLK3 and Familial prostate cancer.