Using the same mouse bacteremia model with intravenous polymyxin B (4 mg/kg, the maximum dose in mice via intravenous administration), the average bacterial count of spoT mutant in blood at 4 h reduced to below the limit of detection of 1.30 log10 CFU/mL, showing at least >1.65 log10 CFU/mL reduction compared to that of the wild-type AB5075 (Fig 7). Here, THEMIS is linked to bacterial infectious disease with sepsis.