CD163 and neoplasm: In order to validate some of the observed changes at the protein level, in vitro–polarized macrophages from multiple healthy donors were analyzed via flow cytometry; NC410 treatment of macrophages polarized with IL-4/IL-13/collagen or tumor-conditioned medium (TCM)/collagen consistently and significantly reduced the expression of CD163 on the cell surface (Figure 7G), while not affecting the expression of CD206 (MRC1, Figure 7H).