Initial transcriptomic analysis for the presence of NC410 and bintrafusp alfa targets on MC38 tumor–infiltrating immune cells within all treatment groups (Figure 2, F and G) revealed high expression of Pdcd1 (encoding PD-1) in T cells, mainly CD8+, and NKT cells, while the gene encoding PD-L1 (Cd274) was expressed in most immune cells, particularly PMNs, M1 macrophages, and cDCs. This evidence concerns the gene PDCD1 and neoplasm.