Here we demonstrate that primary and metastatic colon cancer tissues exhibit a range of collagen content that is bound by NC410, with collagen-rich regions seemingly surrounding epithelial tumor cell islands and overlapping with areas of CD45+ immune cell infiltration, supporting the potential exploration of the combination NC410 plus bintrafusp alfa in patients with advanced colon cancer. This evidence concerns the gene PTPRC and malignant colon neoplasm.