Because viral dependency factors identified via CRISPR screening might be required in a cell type-specific manner, we evaluated the requirement of these genes for infection in multiple cell lines expressing ACE2 (the receptor for both SARS-CoV-2 and HCoV-NL63), specifically A549-ACE2, HT1080-ACE2, and 293T-ACE2. The gene discussed is ACE2; the disease is infection.