CD8+ cell-depletion not only completely abolished the efficacy of anti-PD-1 antibody treatment but also dramatically increased tumor growth (TGI − 78%, Table 1 Experiment 2 and Fig. 3B), suggesting TCTL-TILs plays vital role in the MOA of anti-PD-1 antibody treatment and tumor immunity. Here, CD8A is linked to neoplasm.