One such example was the Treg depletion: the CD25 antibody mediated depletion slowed down MC38 tumor growth, but not Hepa1-6 tumor growth; in addition it enhanced anti-PD-1 efficacy in several tumor types but not Hepa1-6; however, Foxp3+ Treg depletion by engineering/DT treatment enhanced inhibition of Hepa1-6 tumor growth, specifically and persistently, which was explained by the possibility that CD25 antibody also targeted CD4 Teff that were required for Hepa1-6 tumor growth, thus providing additional insight into the tumor immunity. The gene discussed is PDCD1; the disease is neoplasm.