Therefore, in this study, we applied a new digital crush platform to make the CS-AKI mouse model, and to explore the role of the RIG-I/Caspase1/GSDMD signaling pathway in the macrophage polarization and pyroptosis in vivo and in vitro, aiming to elucidate the mechanism of inflammatory response in the CS-AKI model. The gene discussed is GSDMD; the disease is Cowden syndrome 1.