The PLGA (PD-L1 siRNA+PD-1 siRNA)-NPs with vaccination group showed significant inhibition of tumor growth compared with the positive control with vaccination (89% decrease, p<0.001) and anti-PD-L1+anti-PD-1 with vaccination (87% decrease, p<0.001) groups (figure 6B). This evidence concerns the gene PDCD1 and neoplasm.