CD8A and neoplasm: TC-1 cells were derived from primary lung epithelial cells of C57BL/6 mice and expressed HPV16-E6 and HPV16-E7.22 In addition, to extend our concept, we used E7 peptide-incorporated PLGA (E7+poly I:C)-NPs as a therapeutic vaccine to induce an E7-specific CD8+ T cell immune response.31 32 Vaccination with PLGA (E7+poly I:C)-NPs led to an increased antigen-specific immune response, as has been reported previously.22 Seven days after subcutaneous injection of TC-1 tumor cells into C57BL/6 mice, PLGA (E7+poly I:C)-NPs were subcutaneously injected into the mice twice at an interval of 1 week.