Notably, the tumor weight in the PLGA (PD-L1 siRNA+PD-1 siRNA)-NPs with vaccination group was significantly lower than that in the positive control with vaccination (80% decrease, p<0.001) and anti-PD-L1+anti-PD-1 with vaccination (75% decrease, p<0.001) groups (figure 6C, D). This evidence concerns the gene CD274 and neoplasm.