Next, the results of Masson’s trichrome staining (Fig. 1c, P < 0.01) and the expression of fibrosis indicators Col-I, α-SMA, and FN revealed that M-group mice had more severe renal fibrosis than Con-group mice, and CF-L and CF-M significantly attenuated renal fibrosis in SAMP8 mice (Fig. 1e-g, P < 0.01). This evidence concerns the gene ACTA1 and renal fibrosis.