Hence, in this study, the steroid dexamethasone was loaded into self-assembled hyaluronan-cholesterol NHs with the aim to investigate corticosteroid-induced enhanced of transport processes driving bicarbonate excretion in the biliary epithelium (NHE-1 isoform) and to evaluate the effects of dexamethasone-loaded NHs (NHs/dex) on liver injury induced by experimental cholestasis. This evidence concerns the gene SLC9A1 and cholestasis.