EPCAM and hepatocellular carcinoma: To confirm that EpCAM-specific ICE® constructs and control antibodies can effectively target EpCAM-expressing tumor cell lines, EpCAM-specific ICE® constructs and IgG1 control antibodies were titrated at increasing concentrations for binding to the HCC-1187, HCC-1954, and Detroit 562 tumor cell lines that express relatively high levels of EpCAM (Table S4), and cell surface-bound constructs were analyzed by flow cytometry.