Despite having the lowest apparent binding affinity to NK cells, the EpCAM/CD16A ICE® showed superior cytotoxicity compared with that of the EpCAM/NKG2D and EpCAM/NKp46 ICE® constructs against EpCAM+ tumor cells potentially due to higher cell-surface expression levels of CD16A and/or stronger NK cell activation upon CD16A ligation. Here, KLRK1 is linked to neoplasm.