Therefore, in our study PPAR α −/− mice fed with high-cholesterol diet for 8 weeks were used, wherein the cardiac hypertrophy was established and the hypertrophic markers like ANP and BNP also showed an upsurge as expected, apart from these, the Masson Trichome staining revealed significant accumulation of collagen in both PPAR α−/− mice as well as HCD fed PPAR α−/− mice. This evidence concerns the gene PPARA and cardiac hypertrophy.