Notch signaling is activated in podocytes of glomerular diseases (27), and Notch3 has been further shown to be activated and promote crescent formation in glomerulonephritis in animal (28), We therefore examined Notch3 signaling in cultured podocytes treated with HB-EGF, and found it was activated as indicated by increased Notch3 intracellular domain (NICD3) (Figure 6B). This evidence concerns the gene HBEGF and glomerular disorder.