As for GSEA, whole gene expressions were all included in analysis, the results were shown in Figures 2C,D, which suggested that nucleotide excision repair, nucleocytoplasmic transport, ribosome biogenesis in eukaryotes, etc. were enriched in normal group, while neutrophil extracellular traps formation, TNF signaling pathway, toll-like receptor signaling pathway, NOD-like receptor signaling pathway, NF-κB signaling pathway, and chemokine signaling pathway, etc. were enriched in IDD patients. The gene discussed is TNF; the disease is intervertebral disk degenerative disorder.