All 100 CRC probands, who met the previously established criteria, after appropriate genetic counseling, were genetically tested for germline variants in different LS-associated susceptibility genes, such as MLH1, MSH2, MSH6, PMS2 and EPCAM. The mutational screening of the investigated study cohort showed that 59 out of 100 probands carried germline MMR benign/likely benign variants (MMR-w. This evidence concerns the gene PMS2 and colorectal carcinoma.