Lipopolysaccharide (LPS) combined with nigericin was reported to induce pyroptosis (24, 31); our results showed that treatment of LPS and nigericin could induce HCC cell pyroptosis by remarkably upregulating the expression of active Caspase-1 and N-terminal GSDMD which are commonly used markers of pyroptosis. This evidence concerns the gene GSDMD and hepatocellular carcinoma.