To uncover the AFF4-mediated downstream gene contributes to tumor metastasis, quantitative real-time PCR (qRT-PCR) screened the expression of a panel of multiple EMT-related genes, including CDH1, CAV1, ITGB1, CDH2, MMP9, VIM, CLDN3, FN1, and VDR. As shown in Figure 5A, AFF4 depletion significantly induced a decreased expression of CDH1 while other genes, especially CAV1, ITGB1, MMP9, VIM, FN1, and VDR has a slight change in mRNA level (Figure 5A). This evidence concerns the gene CLDN3 and neoplasm.