The use of bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfifide (BPTES) can reduce metabolic intermediates such as thymine and carbamoyl phosphate by inhibiting GLS, which additionally reduces pyrimidine supply with the concomitant use of 5-FU in NSCLC, thereby synergistically inducing cell death (30). This evidence concerns the gene GLS and non-small cell lung carcinoma.