Given the lack of comprehensive studies exploring the TERT behavior and the expression of IC in the same set of BC tumors, we performed a prospective study investigating the hotspot mutations and the methylation status of TERT promoter, gene expression level, and their relationship with telomere length in parallel to the expression of PD1, PD-L1, and PD-L2 in the same cohort of 70 BC patients. The gene discussed is TERT; the disease is breast cancer.