To investigate the biological characteristics shared by the different PLAGL2 expression levels on proliferation, migration, and F-actin polymerization of gliomas, we divided HGG patients from GSE4290 into PLAGL2-positive and PLAGL2-negative groups and performed GSEA, a robust computational method that determines whether an a priori defined set of genes is statistically significant, as well as the concordant differences between both groups. Here, PLAGL2 is linked to glioma.