Since obesity upregulates the expression levels of IL36G in VAT and PBMC and the expression of IL36R is higher in the SVFC compared to adipocytes, we further explored the adipocyte-M1 macrophage crosstalk analyzing the effect of the ACM obtained from patients with obesity in the expression of IL36G, its receptor and CTSG. We observed a tendency to increased expression levels of IL36G, but differences were not statistically significant (Figure 4A). This evidence concerns the gene CTSG and obesity due to melanocortin 4 receptor deficiency.