NET formation in normoxia or hypoxia setups was evaluated by (I) stimulation of isolated human peripheral blood neutrophils, and (II) collection of human tissue samples from acute myocardial infarction and other neutrophil-rich diseases without overt hypoxia through immunohistochemical analysis of HIF-1α and neutrophil elastase (NE). This evidence concerns the gene ELANE and acute myocardial infarction.