This study aimed to observe the effects of Jingyin granule on an in vitro cell model of PF, determining the protein expression levels of Janus kinase 2/signal transducers and activators of transcription (JAK2/STAT3), the mammalian nuclear factor-κB (NF-κB), the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), tumor necrosis factor (TNF), and the extracellular signal-regulated kinases 1 and 2 (ERK1/2), and to investigate the anti-fibrotic mechanism in preventing PF. Here, TNF is linked to pemphigus foliaceus.