The upregulation of GFAP in Müller cells has been previously described in animal models of diabetic retinopathy (Barber et al., 1998), RP (Fernandez-Sanchez et al., 2010), and glaucoma (Fernandez-Sanchez et al., 2013), and in vitro by our research group (Fernandez-Bueno et al., 2008, 2012, 2013; Di Lauro et al., 2016; Labrador-Velandia et al., 2019; Usategui-Martín et al., 2020) by using the same organotypic neuroretina model, our results presented here to add more evidence to this premise. This evidence concerns the gene GFAP and glaucoma.