NFS1 and colorectal carcinoma: To verify the role of NFS1 in oxaliplatin treatment, we treated CRC cells with different concentrations of oxaliplatin and found that NFS1 knockdown exerted a significant synergistic effect with oxaliplatin to notably reduce cell viability (Fig. 1g, h) and increase cell cytotoxicity, as demonstrated through an analysis of lactate dehydrogenase (LDH) activity (Fig. 1i, j).