To evaluate the function of NFS1 and FDX2 in the behaviors of CRC cells, we silenced NFS1 and FDX2 in HCT116 and DLD1 CRC cells (Fig. S1e) and found that NFS1 inhibition exerted a better suppressive effect on cell growth (Fig. 1e and Supplementary Fig. S1f). This evidence concerns the gene FDX2 and colorectal carcinoma.