In addition, the overall survival (OS) analysis demonstrated that Twist1 high expression in gliomas predicted short survival of patients by analyzing the TCGA database (Fig. 6E left & Supplementary Fig. 6, Kaplan-Meier method, p = 0.02 & 0.026, respectively) and CGGA datebase (Fig. 6E right, Kaplan-Meier method, p = 7.12e-09). This evidence concerns the gene TWIST1 and glioma.