Horizontal pleiotropy was observed for the associations of genetically predicted SHBG with hypertension and type 2 diabetes, and for the association of genetically predicted testosterone with type 2 diabetes (P for MR-Egger intercept <0.05, Supplementary Tables S9 and S10, available as Supplementary data at IJE online). This evidence concerns the gene SHBG and type 2 diabetes mellitus.