To better confirm the inhibitory effect of Trim31 on steatohepatitis, the ex vivo gene therapy intervention approach by lentivirus loaded with mouse full-length Trim31 sequences (LV-Trim31) or human full-length Trim31 sequences (LV-hTRIM31) was employed to further evaluate the role of Trim31 on mitigation of liver steatosis, insulin resistance and inflammation (Fig. 4a and Supplementary Fig. S8a). Here, TRIM31 is linked to Hepatic steatosis.