Additionally, in the complicated regulatory network of liver metabolism, in the present study, the increased Rhbdf2–MAP3K7 axis activity and downstream pro-inflammatory events during HFD-triggered liver steatosis were markedly prevented by liver-specific Trim31 overexpression, indicating the inhibitory function of Trim31 on the Rhbdf2-MAP3K7-mediated inflammatory response, insulin resistance, and steatosis. This evidence concerns the gene INS and Hepatic steatosis.