Furthermore, immunofluorescence showed that AURKA and p-Histone H3 overlapped in untreated KYSE150 and KYSE450 ESCC cells; however, treatment with oxethazaine blocked histone H3 phosphorylation, resulting in decreased fluorescence overlap (Fig. 4C–E). The gene discussed is AURKA; the disease is esophageal squamous cell carcinoma.