A previous study reported that melatonin could inhibit RhoA/ROCK pathway activation in choroidal neovascularization.38 To test whether melatonin restored BMAL1 expression and attenuated IDD development through RhoA/ROCK pathway inhibition, we used arachidonic acid (AA), a ROCK agonist that interacts with the ROCK regulatory region, in our experiments. The gene discussed is RHOA; the disease is intervertebral disk degenerative disorder.