Through real-time quantitative polymerase chain reaction (RT-qPCR) with 5 different colon cancer cell lines and the CCD-18Co as the control, we found CCDC12 was relatively overexpressed in LOVO and SW480 cell lines (Fig. S1), hence we reduced their expression levels using sh-CCDC12 RNA. The gene discussed is CCDC12; the disease is colonic neoplasm.