To understand whether the TS-MAP approach included these GBM-microenvironment subsets, we performed immunofluorescence costaining for cell-surface biotinylation and specific markers of immune cells (CD45+), TAMs (CD68+), endothelial cells (CD31+), and EGFR+ cells (SI Appendix, Fig. S4). The gene discussed is CD68; the disease is glioblastoma.