The N-glycosylation of HER2 protein at asparagine (Asn) 68/124/187/259/530/571/549 can inhibit its binding with Herceptin and activate mitogen-activated protein kinase (MAPK) and PI3K-Akt signaling pathways to promote breast cancer progression (Fig. 3B). This evidence concerns the gene AKT1 and breast carcinoma.