To further confirm the mechanistic pathway between CD133, AKT activation, and the pro- and anti-apoptotic BCL-2 family proteins, BAKP, and POT melanoma cells expressing low barely detectable CD133 levels, were co-transduced with a Tet activator (rtTA3) plus a Tet-on vector expressing CD133 (TRE3G-CD133), or with rtTA3 alone, and selected with blasticidin and gentamicin. This evidence concerns the gene AKT1 and melanoma.