Furthermore, obesity induced by a high-fat feeding provoked a crucial decrease in ASAH2 expression (−12.19%, p < 0.05, Figure 5G, vs. control group), which was further elevated by prolonged CBD treatment (+20.03%, p < 0.05, Figure 5G) in comparison to the rats from the HFD group. Here, OPN1MW is linked to obesity due to melanocortin 4 receptor deficiency.