GLIS1 and neoplasm: To investigate the effects of CAF-derived GLIS1 on intraperitoneal tumor metastasis in vivo, we established xenograft tumor models through inoculation of a mixture of SKOV3 cells and CAFs transfected with siGLIS1 or Control siRNA at a 1:4 ratio in the peritoneal cavity of nude mice (Figure 3A).