Sixty-one single-cell colonies were manually isolated and screened for their CGG repeat number in the 5’-untranslated region of FMR1 (Figure 1A) and by the AmplideX PCR/CE FMR1 Reagents (Asuragen, TX, USA) commonly used for clinical diagnosis of FXS (Figure 1B). This evidence concerns the gene FMR1 and fragile X syndrome.