Following infection with either MVA or HSV-1 ΔICP0, the expression of IRF3 and/or NF-κB-dependent Isg56, Cxcl10, and Ifnb were significantly upregulated in WT cells and showed expected kinetics with significantly higher expression at 6 h p.i. in comparison to non-stimulated control cells and 3 h p.i. (Figure 3A,B). This evidence concerns the gene IRF3 and infection.