As shown in Figure 8B, the glutamate receptor, ionotropic, AMPA4 subunit (Gria4) mRNA, which was 1.23-fold up-regulated in PWS mice compared to WT mice in the original RNA-seq data, was not found to be significantly increased with QPCR (p = 0.07). This evidence concerns the gene GRIA4 and Prader-Willi syndrome.