For example, comparing the expression of CD27 and CD28 in the T-cell subsets from healthy subjects, untreated DLBCL patients, and treated DLBCL patients with multiple rounds of chemotherapy, such as with alkylating agents, showed no difference between the healthy subjects and the untreated DLBCL patients, whereas the heavily treated DLBCL patients were double negative for CD27 and CD28 [61]. Here, CD28 is linked to diffuse large B-cell lymphoma.