Several markers can be used to verify the efficacy of a vaccine, but the role of IFN-γ+-producing CD4+ T cells is already well-established in the protective immune response against M. tuberculosis, which is important for the control of bacterial growth in lung tissue [35,36], the restriction of Mtb growth in macrophages [37], the formation of granulomas [38,39], and the regulation of the Th17 profile [40]. Here, CD4 is linked to Granuloma.