In vaccination-protected mice, however, the response to malaria of Runx1 expression was significantly (p-value < 0.05) increased from approximately 18 on day 0 p.i. to approximately 39 on day 1 p.i., and highly significantly (p-value < 0.01) to approximately 42 on day 4 p.i. Maximum expression occurred at peak parasitaemia, before a highly significant (p-value < 0.01) decline towards the end of crisis on day 11 p.i. as compared with unvaccinated mice. This evidence concerns the gene RUNX1 and malaria.